Few life science businesses are started by women despite nearly half of life science graduate students being women. To increase early career women life scientists’ interest and intent in starting a business, we developed and evaluated an online program featuring interactive simulations. To develop the simulation narrative, we identified common entrepreneurship needs of women scientists using literature review, surveys, and focus groups. The common needs we identified included the need for strong networks of support and mentoring, effective communication skills, and better understanding of business, finance, and available resources. To address those needs, we presented an introductory, experiential, narrative overview of starting a business with characters based on real life women who overcame barriers to entrepreneurship. Through the simulations, we aimed to increase the scientists’ awareness of rewarding aspects of entrepreneurship, understanding of what is involved, basic skills needed in the initial phases, and business-related confidence. We evaluated the completed product with a diverse group of 28 women life scientists. After using the program, most of them (>85%) reported an increased intention to engage in activities that help entrepreneurs get started, including doing more networking, seeking mentors, and participating in life sciences organizations for women. Around a quarter of participants (27.3 %) said they were more likely to start a business someday after completing the program.